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Objective:To explore the application of artificial intelligence (AI) assistant system combined with case-based learning (CBL) in the teaching of strabismus specialty for ophthalmic residents.Methods:Forty ophthalmic residents who were trained in the Department of Ophthalmology of the People's Hospital of Peking University from January 2020 to December 2021 were divided into control group and experimental group, with 20 people in each group. The control group used the traditional teaching mode, and the experimental group used AI assistant system combined with CBL teaching. At the end of specialized training, the two groups were assessed for theoretical knowledge, clinical skills, clinical thinking and questionnaire survey, and the teaching effect was evaluated. SPSS 26.0 was used for Chi-square test, one-way ANOVA and independent-samples t-test. Results:The scores of theoretical knowledge [(34.10±1.33) points], clinical skills [(24.75±1.02) points] and clinical thinking [(24.80±0.77) points] in the experimental group were significantly higher than those in the control group [(33.15±1.35) points, t=2.24, P=0.031; (23.60±0.82) points, t=3.93, P<0.001; (24.20±0.83) points, t=2.37, P=0.023]. For the evaluation of teaching effect, the experimental group improved the learning interest ( t=11.47, P<0.001), clinical thinking ability ( t=9.36, P<0.001), knowledge and skill level ( t = 17.71, P < 0.001), knowledge sharing ( t=31.17, P< 0.001) and overall satisfaction ( t=10.60, P<0.001), and these 5 aspects were scored higher in experimental group compared with the control group. Conclusion:The teaching mode combining AI assistant system and CBL teaching can improve the independent diagnosis and treatment ability of ophthalmic residents for strabismus diseases, establish a correct clinical thinking path, and obtain a better teaching effect than the traditional teaching mode.
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Objective:To explore whether chronic fluorosis can cause liver fibrosis in rats by observing expression changes in type Ⅰcollagen (Col-Ⅰ), type Ⅲ collagen (Col-Ⅲ) and alpha smooth actin (α-SMA) in the liver tissue of chronic fluorosis rats.Methods:According to body weight (90 - 100 g), forty-eight SD rats were randomly divided into control group (drinking water fluoride ion concentration < 0.5 mg/L), low, medium and high concentration fluoride groups (drinking water fluoride ion concentration of 5.0, 50.0 and 100.0 mg/L), with 12 rats in each group (half male and half female), and fed for 6 months. Fluoride ion selective electrode method was used to detect bone fluoride and urinary fluoride levels; hematoxylin-eosin staining (HE staining) and Masson staining were used to observe the pathological and morphological changes and the collagen deposition of liver tissue; quantitative real-time polymerase chain reaction and immunohistochemical staining were used to observe Col-Ⅰ, Col-Ⅲ and α-SMA mRNA and protein expressions.Results:There was significant difference in bone fluoride and urine fluoride between the 4 groups [bone fluoride: (92.52 ± 5.64), (112.21 ± 11.86), (142.99 ± 7.87), (235.63 ± 11.55) mg/kg; urinary fluoride: (5.47 ± 0.88), (17.78 ± 1.48), (54.16 ± 5.96), (121.11 ± 6.32) mg/L, P < 0.001]. Under light microscope, with the increase of fluoride concentration, the degree of hepatic cell edema was aggravated, and the deposition of collagen fiber around the central vein and the portal area increased significantly. The mRNA expression level of Col-Ⅰ in low, medium and high concentration fluoride groups (1.20 ± 0.09, 1.80 ± 0.08, 1.58 ± 0.06) was significantly higher than that in control group (1.00 ± 0.00, P < 0.05); Col-Ⅲ and α-SMA mRNA expression levels in medium and high concentration fluoride groups (Col-Ⅲ: 1.15 ± 0.14, 1.64 ± 0.24; α-SMA: 1.69 ± 0.02, 2.34 ± 0.06) were significantly higher than those of low concentration fluoride group (Col-Ⅲ: 0.59 ± 0.17; α-SMA: 0.80 ± 0.13, P < 0.05). With the increase of fluoride concentration, the liver tissue Col-Ⅰ(0.00 ± 0.00, 0.03 ± 0.01, 0.08 ± 0.01, 0.13 ± 0.02), Col-Ⅲ (17 803.05 ± 3 221.16, 47 523.15 ± 3 490.10, 127 786.35 ± 13 008.86, 237 233.03 ± 47 614.63) and α-SMA (516.83 ± 181.18, 2 885.03 ± 864.92, 11 186.94 ± 2 394.08, 37 182.43 ± 12 390.59) protein levels were also increased significantly ( P < 0.05). Conclusion:Long-term excessive intake of fluorine may cause the production of collagen fibers around the central vein and the portal area of the liver in rats to increase, and then lead to the formation of liver fibrosis.
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Objective To analyze the infection situation and risk factors of Helicobacter pylori (Hp) in children in Yulin, Shaanxi province. Methods A total of 374 preschool children with digestive tract symptoms in Yulin area from December 2019 to December 2020 were selected. All the research object of personal data by using self-designed questionnaire (gender, age), personal experiences and health habits (common tableware, common koubei brushing your teeth, wash your hands before the rice then empress), the life habits (whether diet, koubei utilities, brushing your teeth regularly to suck finger), socioeconomic status, place of residence, with the number of living, family economic income), caregivers has a history of stomach problems (parents, nanny) And so on. The investigation method is the combination of telephone inquiry and on-site investigation. 3ml fasting venous blood was collected, and the supernatant was centrifuged. Serum IL-17 and IL-10 levels were determined by ENZYme-linked immunosorbent assay, and Treg and Th17 cell levels were determined by flow cytometry. Hp infection was detected by 14C urea breath test. Univariate analysis and logistic regression were used to analyze the independent risk factors of Hp infection in children. Results Eighty-three of the 374 children were infected with Hp, and the infection rate was 22.19%. The levels of Treg and IL-10 in peripheral blood of Hp infected children were significantly lower than those of non-hp infected children (P<0.05). The levels of Th17 and IL-17 in peripheral blood of children with Hp infection were significantly higher than those without Hp infection (P<0.05). In univariate analysis, there were statistically significant differences between the two groups in age, parental education level, per capita monthly income, sharing tableware, sharing toothbrushing cups, washing hands before and after meals, sucking fingers, parents' knowledge of Hp, and caregivers' history of stomach disease (P<0.05). Logistic regression analysis showed that sharing tableware, sharing toothbrushing cups, sucking fingers, parents' lack of knowledge about Hp, and caregivers' history of gastric diseases were independent risk factors for Hp infection in children (P<0.05). Conclusion The infection rate of Hp in children is high, and Hp infection can cause immune system dysfunction in children. It is necessary to take targeted and effective clinical measures to prevent Hp infection.
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Objective:To evaluate and compare the foveal microvascular morphology and central foveal thickness (CFT) after laser retinal photocoagulation and intravitreal injection of anti-vascular endothelial growth factor (VEGF) in patients with retinopathy of prematurity (ROP), and to explore the factors affecting the prognosis of vision.Methods:A cohort study was conducted.Forty children (40 eyes) aged 4-6 years, who had been treated in Peking University People's Hospital for type 1 ROP from January 2019 to December 2020, were enrolled.Optical coherence tomography angiography (OCTA), best corrected visual acuity (BCVA) and refractive status of the patients were examined.The patients were divided into laser retinal photocoagulation group and anti-VEGF group according to they received a single laser retinal photocoagulation therapy or a single intravitreal injection of anti-VEGF drugs (conbercept or ranibizumab 0.25 mg/0.025 mL) after birth.Twenty age-matched full-term healthy children (20 eyes) were enrolled as the normal control group.The FAZ area, superficial and deep foveal vessel density (VD) and CFT of the affected eyes were measured by OCTA at 4-6 years after treatment to investigate the influence of gestational age, birth weight, morphological characteristics of foveal microvessels and CFT on the prognosis of BCVA.This study protocol adhered to the Declaration of Helsinki and was approved by an Ethics Committee of Peking University People's Hospital (No.2017PHB179-01). Written informed consent was obtained from the guardians prior to any medical examination.Results:There were statistically significant differences in FAZ area, superficial foveal VD and deep foveal VD among the three groups ( F=12.321, 8.436, 5.497; all at P<0.05). The FAZ area was smaller, and the superficial and deep foveal VD of the laser photocoagulation group and the anti-VEGF group were greater than those in the normal control group, and the difference was statistically significant (all at P<0.05). The CFT of the laser photocoagulation group was (267.6±11.8)μm, greater than (259.5±12.9)μm of the anti-VEGF group and (242.4±12.3)μm of normal control group, and the CFT value of the anti-VEGF group was greater than that of the normal control group, and the differences were statistically significant (all at P<0.05). There was a strong negative correlation between the superficial foveal VD and FAZ area ( r=-0.713, P<0.05), a moderate negative correlation between the deep foveal VD and FAZ area ( r=-0.565, P<0.05), and a moderate positive correlation between gestational age and FAZ area ( r=0.485, P<0.05). Pearson correlation analysis results showed that gestational age, FAZ, superficial foveal VD, deep foveal VD, CFT were all correlated with BCVA (all at P<0.05). The effects of gestational age and FAZ on BCVA were both statistically significant ( R2=0.615, both at P<0.05). Conclusions:The morphology of microvessels in macular fovea and the prognosis of BCVA in the affected eye is similar at 4-6 years after laser retinal photocoagulation and intravitreal injection of anti-VEGF drugs for type 1 ROP.The CFT of the affected eye after anti-VEGF drug therapy is better than those after laser retinal photocoagulation.Gestational age and FAZ are the influencing factors of visual acuity after treatment in children with type 1 ROP.
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Objective:To explore the application effects of work record analysis combined with emergency plan drill on the practice teaching of nursing students in department of emergency.Methods:A total of 80 nursing students who had clinical practice in the Department of Emergency of Beijing Shijitan Hospital Affiliated to Capital Medical University between February 2018 and August 2020 were selected as research subjects. They were divided into control group and observation group according to the admission order to the department. The control group was given routine teaching, and the observation group adopted work record analysis combined with emergency plan drill teaching. The theoretical knowledge level, the success rate of emergency treatment and teaching effect evaluation were compared between the two groups of nursing students before and after learning. SPSS 19.0 was used for t test and chi-square test. Results:After learning, the scores of theoretical knowledge (pre-hospital emergency, common emergency diseases, emergency treatment plan, and complications) of the two groups were higher than those before learning, and the scores in observation group were higher than those in control group ( P<0.05). The success rates of emergency treatments (electrocardiogram, electrocardiogram monitoring, blood sample collection, thrombolytic therapy coordination, and cardiopulmonary resuscitation) and satisfaction rates of teaching effects (fully understanding emergency work, improving the ability of independent learning, enhancing the ability of flexible use of knowledge, and active classroom atmosphere) were higher in observation group than control group ( P<0.05). Conclusion:Work record analysis combined with emergency plan drill can effectively ensure the teaching effects of nursing students in department of emergency, and has the advantages of improving the theoretical knowledge and practical operation ability.
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Phosphorylation is the most common and important post-translational modification of proteins, which plays an important role in the regulation of cell proliferation, differentiation, development and metabolism, and is closely related to the tumorigenesis and metastasis of cancer. Protein kinases and phosphatases generally regulate protein phosphorylation levels as a pair of opposite acting enzymes. Protein phosphorylation in eukaryotes occurs mainly in serine, threonine, and tyrosine residues, and their roles in tumorigenesis and development have been extensively studied. But the roles on histidine phosphorylation is less known due to the immature mass spectrometry and enrichment techniques. In recent years, with the rapid development of related technologies and the discovery of new histidine phosphatases, researchers have paid more attention to the roles of histidine phosphorylation in tumors. Therefore, we aim to review the roles of histidine kinases and phosphatases in tumor. .
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Objective:To investigate the effects of anesthesia depth control on cognitive function and high mobility group protein B1 (HMGB-1) level in older adult patients with breast cancer.Methods:Eighty-six female older adult patients with breast cancer who received mastectomy between June 2019 and June 2020 in the First Hospital of China Medical University, China were included in this study. They were randomly assigned to undergo anesthesia with sodium phenobarbital and atropine at deep (bispectral index 30-45, deep anesthesia group, n = 43) or superficial level (bispectral index 45-60, superficial anesthesia group, n = 43). The mean arterial pressure, heart rate, HMGB-1 level, Mini-Mental State Examination (MMSE) score were assessed in each group. Results:There were no significant differences in mean arterial pressure and heart rate recorded during each time period between the deep anesthesia and superficial anesthesia groups (all P > 0.05). No significant difference in HMGB-1 level was found between the two groups before anesthesia induction and at the end of surgery (both P > 0.05). At 1 and 2 days after surgery, HMGB-1 level in the deep anesthesia group was (75.46 ± 3.33) pg/mL and (93.98 ± 4.32) pg/mL, respectively, which was significantly lower than that in the superficial anesthesia group [(87.89 ± 5.13) pg/mL and (121.01 ± 4.36) pg/mL, t = 13.327, 28.878, both P < 0.05)]. At 1 day before surgery, there was no significant difference in MMSE score between the two groups ( P > 0.05). In the deep anesthesia group, MMSE score was (26.73 ± 1.11) points, (28.16 ± 0.72) points, and (28.97 ± 0.88) points at 1, 3 and 6 days after surgery respectively, which was significantly higher than that in the superficial anesthesia group [(21.03 ± 1.46) points, (22.39 ± 1.24) points, and (24.69 ± 0.57) points, t = 20.380, 26.388, 26.768, all P < 0.05]. Conclusion:Deep anesthesia for mastectomy in older adult patients can reduce cognitive impairment and decrease HMGB-1 level after surgery, and plays a positive role in postoperative recovery.
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Objective:To investigate auricular lobule index difference in healthy young female of different height or weight, and to provide an additional reference for otoplasty of auricular lobule.Methods:Subjects were made up of 266 healthy young females of Henan residents. Their height, weight, physiognomic ear breadth, morphological ear breadth, physiognomic ear length, morphological ear length, auricular lobule length, auricular lobule breadth were measured and calculated. All the data were analyzed by SPSS 15.0 software.Results:We obtained the means, standard deviation of each items, and data were treated statistically. In the 266 subjects, average height was (162.9±4.1) cm, weight (55.3±6.5) kg, morphological ear breadth (4.9±0.6) cm, morphological ear length (2.9±0.3) cm, physiognomic ear breadth (6.2±0.4) cm, physiognomic ear length (3.3±0.3) cm, auricular lobule breadth (1.6±0.3) cm, auricular lobule length (1.8±0.2) cm, lobule physiognomic ear breadth index (25.6±4.9)%, lobule-physiognomic ear length index (53.69±7.69)%, and auricular lobule index (91.4±19.3)%. The differences among the indices mentioned above were of statistical significance ( P<0.05); the indices of subjects of different height were significantly different ( P<0.05); the indices of subjects of different weight were of no statistical difference. ( P>0.05). Conclusions:The subjects of different height or weight have no different morphological ear breadth, morphological ear length, physiognomic ear breadth, physiognomic ear length, auricular lobule length, lobule-physiognomic ear length index and auricular lobule index; hence, the indices are no related to body height and weight. However, the subjects of different height have different auricular lobule breadth and lobule-physiognomic ear breadth index.
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Objective:To investigate the role and mechanism of Nod-like receptor protein 3 (NLRP3) in chronic kidney disease (CKD)-related neointimal hyperplasia (NH) of vessels.Methods:Wild type C57BL/6J male mice were randomly divided into normal control group ( n=6) and experimental group ( n=18), by removal of 5/6 kidney and ligation of left common carotid artery to establish a NH model. After established successfully, the mice in NH experimental group were randomly divided into NH model group, NLRP3 inhibitor group, and drug control group ( n=6/group). C57BL/6J male mice with NLRP3 gene knockout group did not do any treatment after the establishment of NH model. After 3 weeks of feeding, the blood and vascular tissue samples of mice were collected. The pathological changes of vascular tissue samples in mice were observed by hematoxylin-eosin staining. The expressions and localization of NLRP3-related protein were observed by immunofluorescence staining. The expression of NLRP3 mRNA in vascular tissue was detected by quantitative real-time PCR. The activity of caspase-1 in vascular tissue was measured by colorimetric method. Human aortic smooth muscle cells (HASMCs) were treated with 10% uremic serum to simulate the body's internal environment during the uremic phase. NLRP3 small interfering RNA (siRNA) was transfected or NLRP3 inhibitor glibenclamide was added to the cell cultures. The expression of NLRP3 mRNA in HASMCs was detected by quantitative real-time PCR. The activity of caspase-1 in HASMCs was detected by colorimetric method. Results:Compared with the control group, the levels of serum creatinine and blood urea nitrogen were significantly increased in the NH model group (both P<0.01). The vascular histopathology showed that vascular intima thickened, vascular smooth muscle cells proliferated and hypertrophied, nuclei were deeply stained, and cells arranged disorderly and migrated to vascular intima in the experimental group. Quantitative analysis showed that the ratio of neointima to lumen increased significantly in the NH model group than that in control group ( P<0.01). Compared with the control group, the immunofluorescence staining of vascular tissue showed that the expressions of NLRP3, caspase-1, IL-18, IL-1β and proliferating cell nuclear antigen (PCNA) protein in the NH model group increased (all P<0.01), while the expression of α-SMA decreased ( P<0.01). NLRP3 was mainly located in vascular smooth muscle cells (VSMCs). VSMCs showed a synthetic phenotype. Compared with the NH model group, the expression of NLRP3, caspase-1, IL-18, IL-1β and PCNA protein in the NLRP3 inhibitor group and NLRP3 gene knockout group decreased (all P<0.01), the expression of α-SMA increased ( P<0.01), and the pathological changes of blood vessels alleviated. Compared with healthy serum group, the expression of NLRP3, IL-18, and IL-1β and bromodeoxyuridine (BrdU) uptake in uremic serum-stimulated group were increased (all P<0.01). After transfection of NLRP3 siRNA and addition of glibenclamide, the expression of NLRP3, IL-18, and IL-1β in VSMCs in uremic serum-stimulated group decreased, and BrdU intake decreased (all P<0.01). Conclusions:NLRP3 inflammatory bodies play an important role in promoting CKD-related neointimal hyperplasia of vessels, and glibenclamide can effectively reduce neointimal hyperplasia.
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Objective:To observe the protein and mRNA expressions of microtubule-associated protein 1 light chain 3 (LC3)B, P62 and Beclin1 in the liver of rats with chronic fluorosis, and to explore the role of autophagy in pathogenesis of liver injury induced by fluorosis.Methods:Using a group design, 54 SD rats were divided into 9 groups according to their weight (100 - 120 g) using a random number table method, each group with 6 rats, half male and half female. They were control group (NC group), low fluoride group (LF group), high fluoride group (HF group), NC + rapamycin (RAP) group, LF + RAP group, HF + RAP group, NC + chloroquine (CQ) group, LF + CQ group, and HF + CQ group. The NC group drank tap water (fluoride concentration was 0.5 mg/L), LF group drank fluoride water (fluoride concentration was 5.0 mg/L), HF group drank fluoride water (fluoride concentration was 50.0 mg/L); NC + RAP group, LF + RAP group and HF + RAP group were fed with corresponding drinking water, respectively, for 3 months, and then RAP (1.5 mg/kg) was intraperitoneally administered for 10 d; NC + CQ group, LF + CQ group and HF + CQ group were fed with corresponding drinking water, respectively, for 3 months, and then CQ (60 mg/kg) was intraperitoneally administered for 10 d. Bone and 24-hour urine samples of rats in each group were collected to detect the contents of bone fluoride and urine fluoride; liver histomorphological changes were observed through hematoxylineosin staining; protein and mRNA expressions of LC3B, P62 and Beclin1 in liver were detected by immunohistochemistry and real-time fluorescence quantitative PCR, respectively.Results:Compared with the NC group [(0.03 ± 0.00) mg/kg, (0.34 ± 0.08) mg/L], the contents of bone fluoride [(3.86 ± 0.08) mg/kg] and urine fluoride [(1.11 ± 0.16) mg/L] in HF group were higher ( P < 0.05). In the NC group, the lobule structure of liver tissue was clear, the hepatic cords were arranged in order, and the cell structure was normal. There were different degrees of hepatocyte edema in LF and HF groups. After intraperitoneal injection of RAP, compared with the corresponding fluoride group, the morphology of hepatocytes did not change significantly. After intraperitoneal injection of CQ, compared with the corresponding fluoride group, the liver cells showed obvious edema, and the degree of edema aggravated with the increase of fluoride concentration. Compared with the NC group, the protein expressions of LC3B and Beclin1 in HF group were higher ( P < 0.05), and the protein expression of P62 was lower ( P < 0.05). After intraperitoneal injection of RAP, the protein expressions of LC3B and P62 in LF + RAP group was lower than that in LF group ( P < 0.05); Compared with HF group, the protein expressions of LC3B and Beclin1 in HF + RAP group were lower ( P < 0.05). After intraperitoneal injection of CQ, protein expression of P62 in LF + CQ group was higher than that in LF group ( P < 0.05); Compared with HF group, protein expression of P62 in HF + CQ group was higher ( P < 0.05). Conclusions:Early (3 month) fluoride intake could promote autophagy and induce edema of hepatocytes in rats, and RAP had similar effects. CQ may induce liver injury by inhibiting autophagy of hepatocytes.
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Objective:To systematically evaluate the influence of botulinum toxin type A (BTXA) injections in the facial plastic surgery incision.Methods:A systematic literature search for studies which were published on PubMed, EMbase, Cochrane Central, CNKI and VIP, was performed from January 1998 to Octomber 2019. " Facial wound" , " Keloid" , " Scar" , " Cicatrix hypertrophy" , " Botulinum toxin" , " Boutulin" , " Botox" were set as the key words. We included trails related to the influence of botulinum toxin type A (BTXA) injections in the facial plastic surgery incision. The data were extracted and Rev Man 5.3 was used for meta-analysis.Results:Participants included seven randomized controlled trial of 450 patients. The Vancouver scar scale score of BTXA group was higher than those in the control group ( Z=23.92, P<0.01). The visual analogue scale score of BTXA group was higher than that of the control group ( Z=8.60, P<0.01). The scar width of BTXA group was less than that in the control group ( Z=10.84, P<0.01). Patients＇ satisfactory rate of BTXA group was higher than that of the control group ( Z=2.83, P<0.01). Conclusions:The injection treatment of BTXA for facial hyperplastic scar has certain curative effect, without obvious side effects, but the authenticity and stability of therapeutic effect have to be verified by more high quality research.
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Objective:To investigate the association between the polymorphism in a disintegrin and metalloprotease 33 (ADAM 33) gene and keloid in the Chinese Han population from Henan Province. It may provide a new theoretical basis for the prevention and treatment of keloids at the genetic level.Methods:There were 84 patients with keloid and 78 patients with hypertrophic scar in this group.We selected 3 SNPs in ADAM33 gene and detected the sample genotypes by Mass ARRAY system to compare genotype and allele frequency differences at each locus.Results:There were no significant differences in gender and age of two groups ( P>0.05). The genotypes of 3918394 (L1) and rs574174 (ST+ 7) locus in the two groups of ADAM33 gene were consistent with the Hardy-Weinberg genetic equilibrium law ( P>0.05), while the genotypes of rs597980 (ST+ 5) locus in the two groups of ADAM33 gene were not consistent with the Hardy-Weinberg genetic equilibrium law. There was a significant difference of genotypes or alleles in L1 locus between keloid and hypertrophic scar groups ( P<0.05). No significant differences were detected of genotypes or alleles in ST+ 7 locus between two groups ( P>0.05). In addition, there was a significantly difference of GG genotype and G allele between keloid and hypertrophic scar groups ( χ2=6.387、6.117, all P<0.05). There was no significantly difference of all genotypes and alleles in ST+ 7 locus between two groups ( P>0.05). There was weak linkage disequilibrium between ST+ 7 and L1 locus in ADAM33 gene. Haplotype analysis showed that haplotype Hap2 (CA) in keloid group was much lower than hypertrophic scar group ( χ2=6.752, P<0.05). Conclusions:The polymorphism of the L1 locus in the ADAM33 gene may have significant associations with keloid in the Chinese Han population from Henan Province, while the ST+ 7 locus may have no significant associations with that. The GG genotype and G allele of L1 locus is a risk factor for keloids and increases the risk of keloids. The susceptible and protective haplotypes of keloid may exist in Chinese Han population from Henan Province.
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Objective:To investigate the value of CT radiomics mode in differential diagnosis of benign and malignant thyroid nodules.Methods:The clinical and imaging data of 179 patients with thyroid nodules confirmed by pathology from May 2017 to August 2018 were retrospectively analyzed in the Affiliated Huaian First People′s Hospital of Nanjing Medical University. Among the patients, 89 cases were benign nodules and 90 cases were malignant nodules. All patients underwent unenhanced and enhanced CT scan before operation. The stratified random sampling method was used to divide patients into a training group (143 cases) and a testing group (36 cases) according to a ratio of 8∶2. The A.K software was used to extract 378 imaging omics features based on preoperative CT images, and then Spearman correlation analysis and least absolute shrinkage and selection operator regression analysis were used for feature selection and model construction. The receiver operating characteristic (ROC) curve was used to verify the model in the training group and the testing group, and the efficacy of imaging omics features to predict benign and malignant thyroid nodules was evaluated.Results:After feature screening, 16 radiomics features were used to construct an identification model between benign and malignant thyroid nodules. In the training group, the area under the ROC curve (AUC) was 0.92 [95% confidence interval (CI): 0.88-0.97], the sensitivity and specificity were 88.7%, 82.0%, and the diagnostic accuracy of the model was 91.1%. In the testing group, AUC was 0.90 (95 %CI: 0.81-0.98), sensitivity and specificity were 88.5%, 84.6%, and the diagnostic accuracy of the model was 88.2%. Conclusion:The CT radiomics mode has a good diagnostic performance in the identification of benign and malignant thyroid nodules.
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Objective To investigate the relationship between the facial morphology and the nasal shape for young women of Han nationality in Henan.Methods Aiming at the female conscripts in Henan province in 2016,210 healthy young females were enrolled in this study,and their anthropometric features were measured including height,weight,total head height,physiognomic facial height,morphological facial height,upper lip height,lip height,facial width,mandibular width,mouth breadth,nasal height,nasal length,nasal depth,and width of nose.The nasal indexes were divided into 5 groups according to different facial types.And their mean and standard deviation were calculated.The differences were analyzed by SPSS version 21.0.Results Among the indexes measured,there were significant differences in height,face height,shape face height,upper lip height,face width,mandibular width,head height/face width,face height/face width,shape face height/face width and nose index;P values were significant.The classification of facial types was based on the ratio of morphological surface index to area width,and the main types of the groups were mid-facial and narrow-facial types.The differences among different facial types were statistically significant (F =12.75,P＜0.05).The scatter plot showed that there was a linear correlation between nasal index and facial shape.Linear regression analysis showed that the linear equation Y=135.84-75.5X (X is the ratio of morphological index to facial width,Y is the nasal index).Conclusions Nasal type is related to the face type.The linear fitting equation between morphological facial height/facial width and nasal index is Y=135.84-75.5x.These findings may enrich the external nasal database,provide reference for the correction of nasal deformity and nasal reconstruction,and direct the clinical therapy.
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Objective To analyze the correlation between vitamin D levels and vascular endothelial function and plasma renin activity (PRA) in hypertensive patients.Methods 415 patients with essential hypertension admitted to our hospital from January 1 to December 2017 were selected as the experimental group.142 healthy subjects who received physical examination and met the included indicators were selected as the control group.The blood biochemical indexes and the 25 (OH) D contents in the blood of two groups were detected and analyzed.The experimental group was divided into vitamin D mild deficiency group (15-30 mmol/L),severe deficiency group [25 (OH) D ＜ 15 mmol/L] and normal group [25 (OH)D ＞ 30 mmol/L] according to vitamin D content.The endothelial function (RHI value) and renin were compared in the three groups,and multiple linear regression analysis was performed on endothelial function and orthotopic renin activity and related factors.Results The diastolic blood pressure,vitamin D content and blood lipid biochemical indexes of the experimental group and the control group were significantly different (P ＞ 0.05).The higher the vitamin D content,the lower the plasma renin activity PRA;the RHI value of the vitamin D severe deficiency group was lower than the vitamin D normal group [(1.4 ±0.15)mmol/L vs (1.6 ± 0.20) mmol/L] (P ＜ 0.05).Vitamin D was negatively correlated with 24 hours systolic blood pressure (24 h SSD),the standard deviation of 24 hours diastolic blood pressure (24 h DSD),the Standard deviation of daytime systolic blood pressure (dSSD),the Standard deviation of daytime diastolic blood pressure (dDSD),the Standard deviation of nighttime systolic blood pressure (nSSD),the Standard deviation of nighttime diastolic blood pressure (nDSD) (P ＜ 0.05),and positively correlated with the decrease of night systolic blood pressure and night diastolic blood pressure.Vitamin D and total cholesterol were independent influencing factors of endothelial function (P ＜ 0.01).Vitamin D and 24 h mean heart rate were independent influencing factors of orthostatic renin activity (P ＜ 0.01).Conclusions The low vitamin D content in patients with essential hypertension affects endothelial function and plasma renin activity,which should be paid more attention in clinical practice.
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Objective:To investigate the efficacy and safety of bevacizumab combined with chemotherapy in patients with retreated advanced non-squamous non-small cell lung cancer (NSNSCLC) and analyze its prognostic factors. Methods:Forty-one patients with previously treated advanced NSNSCLC in Beijing Chest Hospital from February 2013 to June 2017 were recruited. Clinical data of the patients were retrospectively analyzed. There were 38 cases of adenocarcinoma and 3 cases of other pathological types. Bevacizumab combined with chemotherapy served as second-line treatment for 19 patients, and it served as beyond second-line therapy for 22 pa-tients. Eighteen patients harbored epidermal growth factor receptor (EGFR) gene mutations, while the other 23 patients harbored wild-type EGFR gene. The efficacy and safety of bevacizumab combined with chemotherapy were evaluated. To evaluate the prognos-tic factors, single and multiple factor analyses were conducted. Results:All patients received bevacizumab combined with chemothera-py and could be evaluated for response. The mean number of cycles of chemotherapy and chemotherapy combined with bevacizumab were 3.1 and 5.0, respectively. The objective response rate (ORR) of all recruited patients was 12.2％. The disease control rate (DCR) was 82.9％. Regarding the effect of second-line and beyond second-line therapy in patients, data were similar. The ORRs were 10.5％and 13.6％, respectively (P=0.572), and DCRs were 89.5％and 77.3％, respectively, without significantly statistical difference (P=0.271). The median progression-free survival (PFS) and overall survival (OS) were 4.6 months [95％confidence interval (CI) 3.619-5.581] and 11.9 months (95％CI 9.797-14.003), respectively. In the single factor analysis, patients with EGFR mutations, those who received>4 cy-cles of bevacizumab administration, and women had longer OS (χ2=19.673, P=0.000;χ2=6.820, P=0.009;andχ2=6.374, P=0.012;respec-tively). The Cox regression analysis showed that EGFR mutation status and number of cycles of bevacizumab administration were inde-pendent prognostic factors [hazard ratio (HR)=0.129, P=0.001 and HR=0.336, P=0.012;respectively]. The common adverse reactions in-clude bone marrow suppression, bleeding, hypertension, and proteinuria. Most of them were grade 1-2. Conclusions:Bevacizumab combined with chemotherapy provides good efficacy and controllable safety in patients with retreated advanced NSNSCLC. Patients with EGFR mutations and>4 cycles of bevacizumab administration have superior prognosis.
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Objective To analyze transmission factors of norovirus outbreaks in Guangdong province during 2008-2015 and provide evidence for the prevention and control of norovirus infection.Methods Epidemiological analysis was performed on the data of norovirus outbreaks reported in Guangdong from January 1,2008 to December 31,2015,which were obtained from the Public Health Emergency Management Information System of Guangdong province.The samples collected from the norovirus outbreaks were detected for norovirus by RT-PCR and the gene sequencing of the positive PCR products were performed.Results A total of 96 norovirus outbreaks were reported in Guangdong during 2008-2015.Sixteen outbreaks were reported during 2008-2012and 80 outbreaks were reported during 2013-2015 (83.3％).Eighty-two outbreaks (85.4％) occurred in schools.The infection routes included foodborne transmission in 39 outbreaks (40.6％),person to person transmission in 23 outbreaks (24.0％) and waterborne transmission in 8 outbreaks (7.3％).The gene sequencing results showed that variant G Ⅱ.4/Sydney2012 was the predominant pathogen for 6 of the 20 outbreaks (30.0％) during 2012-2013.Variant G lⅡ.17 was the predominant pathogens for 33 of the 53 outbreaks (62.3％) during 2014-2015.Conclusion The norovirus outbreaks in Guangdong during 2008-2015 were caused by foodborne and person to person transmissions of two emerging variant:G Ⅱ.4/Sydney2012 and G Ⅱ.17.
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Objective To analyze transmission factors of norovirus outbreaks in Guangdong province during 2008-2015 and provide evidence for the prevention and control of norovirus infection.Methods Epidemiological analysis was performed on the data of norovirus outbreaks reported in Guangdong from January 1,2008 to December 31,2015,which were obtained from the Public Health Emergency Management Information System of Guangdong province.The samples collected from the norovirus outbreaks were detected for norovirus by RT-PCR and the gene sequencing of the positive PCR products were performed.Results A total of 96 norovirus outbreaks were reported in Guangdong during 2008-2015.Sixteen outbreaks were reported during 2008-2012and 80 outbreaks were reported during 2013-2015 (83.3％).Eighty-two outbreaks (85.4％) occurred in schools.The infection routes included foodborne transmission in 39 outbreaks (40.6％),person to person transmission in 23 outbreaks (24.0％) and waterborne transmission in 8 outbreaks (7.3％).The gene sequencing results showed that variant G Ⅱ.4/Sydney2012 was the predominant pathogen for 6 of the 20 outbreaks (30.0％) during 2012-2013.Variant G lⅡ.17 was the predominant pathogens for 33 of the 53 outbreaks (62.3％) during 2014-2015.Conclusion The norovirus outbreaks in Guangdong during 2008-2015 were caused by foodborne and person to person transmissions of two emerging variant:G Ⅱ.4/Sydney2012 and G Ⅱ.17.
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Objective To investigate auricle index difference in Henan residents,and to provide an additional reference for otoplasty.Methods Subjects were made up 266 healthy young of Henan residents.Their height,weight,total head height,maximum head length,maximum head breadth,vertex to tragion height,physiognomic ear breadth,morphological ear breadth,physiognomic ear length and morphological ear length were measured and calculated.All the data were analyzed by SPSS 15.0 software.Results We obtained the means,standard deviation of each items,and data were treated statistically.Height (162.9±4.1) cm,weight (55.3±6.5) kg,total head height (18.6±1.0) cm,maximum head length (17.0±0.7) cm,maximum head breadth (14.1±0.8) cm,vertex to tragion height (11.9±0.7) cm,physiognomic ear length (6.2±0.4) cm,morphological ear breadth (4.9±0.6) cm,physiognomic ear breadth (3.3±0.3) cm and morphological ear length (2.9±0.3) cm were determined.The differences among the indices mentioned above were of statistical significance (P＜0.05);the indices of female subjects of different height were significantly different (P＜0.05);the indices of female subjects of different weight were also statistically different (P＜0.05).The auricle index subjects of different height and weight were of no stistical difference.(P＞0.05).Conclusions Subjects of different height and weight have different total head height;hence,the indices are related to body height and weight.Subjects of different height have different vertex to tragion height,length-height index of the head and breadth-height index of the head.This study has certain reference values on analyzing the characteristics of the human and auricle cosmetology of henan young females.
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Sleep deprivation (SD) has been associated with an increased morbidity and mortality of coronary heart disease (CHD). SD could induce autonomic nervous dysfunction, hypertension, arrhythmia, hormonal dysregulation, oxidative stress, endothelial dysfunction, inflammation and metabolic disorder in CHD patients. This paper reviewed the study results of SD in clinical trials and animal experiments and concluded that SD was associated with cardiovascular risk factors, which aggravated CHD in pathogenesis and outcomes.